[20]. Furthermore, recombination systems such as Cre-lox are widely used, mostly in eukaryotes. Do not sell or share my personal information, 1. US Patent for Soybean cultivar 4826008 Patent (Patent # 11,589,543 NIH Blueprint for Neuroscience Research has created several hundreds of Cre driver mouse lines which are currently used by the worldwide neuroscience community. Because the controls will not have Cre recombinase, any phenotypic differences between them and the Cre/lox mice should be due to the deleted gene in the Cre/lox mice. Cre is a 38 kDa recombinase protein from bacteriophage P1 that catalyzes recombination between LoxP sites. (<U>G</U>ene-<U>A</U>uto-<U>E</U>xcision <U>B</U>inary <U>S</U>ystemGAEBS)(transcription system) . Introduction Cre-lox recombination Cre-lox system- Cre recombinase , loxP site FLP-FRT recombination FLP-FRT system- FLP recombinase , FRT site Mechanism of Cre-lox and FLP-FRT recombination Binding Synapsis , cleavage and strand exchange Three type of arrangement Inversion Translocation/ Insersion Deletion Application of Cre-lox and FLP-FRT recombination Disadvantage of FLP-FRT Advantage and disadvantage of Cre-lox Conclusion References. See, for example, Lyznik et al., Site-Specific Recombination for Genetic Engineering in Plants, Plant Cell Rep, 21:925-932 (2003) and WO 99/25821, which are hereby incorporated by reference. Deletion: If the sites face in the same direction, the sequence between the loxP sites is excised as a circular piece of DNA (and is not maintained). Clipping is a handy way to collect important slides you want to go back to later. . Now, one point of crossover has occurred, which is what is called a Holliday Intermediate. The result of recombination depends on the orientation of the loxP sites. And, it can be used to turn transgene expression on or off, track individual cells or cell lineages (as in theBrainboworConfettimice), generateinversionsortranslocations, and report gene expression. [13] Mechanism of translocation [ edit] An important application of the Cre-lox system is excision of selectable markers in gene replacement. Faust TE, Feinberg PA, O Connor C, Kawaguchi R, Chan A, Strasburger H, Masuda T, Amann L, Knobeloch KP, Prinz M, Schaefer A, Schafer DP.